Everest Biolabs
Ascent Instrument는 Apex SEC(Size Exclusion Column) 컬럼을 최대 8개까지 장착하여 자동으로 Isolation을 해주는 시스템으로서 세포밖소포(Extracellular Vesicle) 및 엑소좀(Exosome)의 손쉽고 순도 높은 분리가 가능합니다.
Summit Instrument는 48 wells Altitude plate를 장착하여 샘플 로딩(Sample Loading) 및 세포밖소포의 분리 정제(EV isolation)와 농축(Concentration)을 전자동으로 수행하는 시스템입니다.
Atlas ELISA Kit 및 Atlas ELISA Reader를 사용하면 50μL 샘플만으로 한 번에 8개 wells 또는 96 well plate 전체를 한 번에 분석할 수 있습니다.
Atlas HSA ELISA Kit
Atlas Human EV ELISA Kit
Atlas Lumi Homebrew Kit
Atlas Lumi Human EV ELISA Kit
Atlas ELISA Reader
Apex SEC Columns
1. Apex MM
Apex MM SEC Columns
High performance isolation
Reproducible performance < 5% CV
Sample volume 0.5-1.0 mL
Combines SEC and MM(multi-mode) resins for superior EV isolation
Validated in culture, plasma, urine, serum & CSF
Apex MM: More than just SEC
By combining SEC with a multi-mode (MM) resin, Apex MM columns significantly enhance EV purity by removing more lipoproteins than SEC alone.
By providing superior EV purity, maintaining optimal yield without introducing size biases, and preserving EV functionality, Apex MM columns significantly enhance biomarker discovery and therapeutic research applications
Optimized for maximum EV purity in plasma/serum samples—optimal for unbiased assays like mass spec and flow cytometry.
Apex MM performance with plasma
◼︎ Apex MM: Optimized for maximum EV purity in plasma/serum samples—optimal for unbiased assays like mass spec and flow cytometry.
| Column Type | Yield | Purity (HSA) | Purity (ApoA1) | Purity (ApoB-100) | Pore Size |
|---|---|---|---|---|---|
| Apex MM | 1.5 | 2.2 | 1.1 | 1.8 | < 50 nm |
| Competitor | 1.0 | 1.0 | 1.0 | 1.0 | 70 nm |
Definitions :
Yield = EVs in fractions 1–3, normalized to Competitor
Purity (HSA) = Yield/HSA, normalized to Competitor
Purity (ApoA1) = Yield/ApoA1, normalized to Competitor
Purity (ApoB-100) = Yield/ApoB-100, normalized to Competitor
Plasma Sample
NTA
ONI
Technical information
The Apex MM column purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
Everest Biolabs Atlas EV and human serum albumin (HSA) ELISA kits can be used to optimize EV yield and purity during fraction collection.
| Column volume | 9.0 mL |
| Input sample volume | 0.5 - 1.0 mL |
| Sample types | plasma, serum, urine, CSF, cell culture media |
| Resin types | cross-linked agarose plus multi-mode |
| Column reproducibility (CV) | 5% |
2. Apex 4B & 6B
Apex 4B and 6B SEC Columns
High performance isolation
Reproducible performance < 5% CV
Sample volume 0.5-1.0 mL
Available with multiple resins
Validated in culture, plasma, urine, serum & CSF
Apex SEC plates
Scale your isolation
Get the same results as Apex columns in a plate format.
Compatible with the Summit instrument
Apex SEC Working Principle
Size Exclusion Chromatography (SEC) is a commonly used method for extracellular vesicle (EV) and exosome isolation from biological fluids. SEC is easy, reproducible, and provides a high yield of purified EV’s that retain their functionality.
Apex SEC works the same whether you are using a column or plate format.
How do I apply Apex columns to my research?
◼︎ Apex 4B: Optimized for balanced purity and yield in plasma—well-suited for targeted assays (immunoassays, imaging, Western blot, pull-downs).
◼︎ Apex 6B: Optimized for maximum EV recovery—best for samples with low-containments like serum-free cell culture media, CSF, and urine.
◼︎ integration note: Apex SEC columns work with the Ascent instrument for improved reproducibility and throughput. Apex SEC plates are designed for full automation on the Summit instrument.
Apex 4B and 6B performance with plasma
◼︎ Apex 4B: Optimized for high purity and lowest protein co-elution (ideal for biofluids)
◼︎ Apex 6B: Optimized for highest EV yield (ideal for cell cultures)
| Column Type | Purity | Yield | Pore-Size |
|---|---|---|---|
| Apex 6B | 1.0 | 1.0 | 20 nm |
| Apex 4B | 5.0 | 0.8 | 35 nm |
| Competitor | 5.0 | 0.3 | 70 nm |
Definitions :
Yield = EVs in fractions 1–3, normalized to Apex 6B
Purity = Yield/HSA, normalized to Apex 6B
Apex maintains performance with reuse
Apex maintains performance and drip speed after 5 reuses. Columns were tested by running 0.5 mL of human plasma sample, and pooling fractions 1–3, which represent the EV fractions.
Columns were washed with 4 mL of NaOH, followed by 17 mL of 1X PBS between reuses. EVs and HSA were quantified using the Atlas ELISA
Apex 4B and 6B isolate the same EVs
EV composition and size comparison. Super resolution imaging (ONI) and size comparison by NTA and TEM show that Apex 4B and 6B purify EVs with the same tetraspanin composition and size.
Cell culture Sample
NTA/TEM
ONI
Plasma Sample
NTA/TEM
ONI
Technical information
The Apex size exclusion column (SEC) purifies extracellular vesicles (EVs) from biological fluids such as plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
Everest Biolabs Atlas EV and human serum albumin (HSA) ELISA kits can be used to optimize EV yield and purity during fraction collection.
| Column volume | 8.75mL |
| Input sample volume | 0.5 - 1.0 mL |
| Sample types | plasma, serum, urine, CSF, cell culture media |
| Resin types | 4 % or 6 % cross linked agarose beads |
| Exclusion limit | 35 nm or 20 nm |
| Column reproducibility (CV) | 5% |
| Format | Single column or 24-well plate |
Ascent Instrument
Ascent Instrument
Automated, high-throughput EV isolation with SEC columns
Run up to 8 columns in parallel
Collect up to 12 fractions per column
Wash up to 8 columns
Compatible with different column types
Programmable fraction collection sequences
Programmable fraction collection
Optimize your fraction collection for your application.
You may adjust these values for your application to work with different column types, between 1 and 8 columns, wash volumes, number of fractions to collect, and the fraction volume.
Apex SEC Columns
High performance isolation
Reproducible performance < 5% CV
Sample volume 0.5-1.0 mL
Available with SEC and multi-mode resins
Validated in culture, plasma, urine, serum & CSF
Compatible with Ascent
Ascent Instrument Demonstration Video
Watch our comprehensive demonstration of the Ascent Instrument, showcasing its automated, high-throughput EV isolation capabilities using SEC Columns.
Apex column Loading
Remove the top green cap from the Apex Column and place the column in the Apex Column rack. You can remove the bottom cap at this time.
Loading Tubes
Load the tubes into the tube rack according to the number of columns being run and the fractions being collected. Ensure that the cap of the tubes is seated properly in the tube rack to avoid any interference with the instrument
Tall column Rack Install
Place the tube rack on top of the tube rack platform and then insert into the instrument. The tube rack should snap into position but ensure it is fully seated.
Technical information
The Ascent instrument is an automated high-throughput fraction collector for isolation of extracellular vesicles (EVs) with SEC columns.
It can operate up to 8 columns simultaneously and is compatible with Apex columns as well as other brands. The programmable fraction collection allows you to optimize collection for any application.
| SEC column capability | Apex 4B Apex 6B Apex MM Pack your own |
| Compatible with qEV™ SEC columns (qEV™ is a trademark of Izon Science®; not affiliated). |
qEVoriginal qEV1 qEVsingle |
| Dimensions (WxDxH) | 397mm x 364mm x 331mm 15-5/8” x 14-5/8” x 13” |
| Weight | 13.6 Kg / 30.0 lbs. |
| Input Power | 100~240 VAC (50-60Hz) |
| Power Supply Unit | 24 Vdc, 2.1A, 50W |
| Environmental Temperature | 10°C -24°C (50°F-75°F) |
| Relative Humidity | 30-80% RH |
| Bottle Capacity | 1000mL |
| Connectivity | USB-C (for software updates) |
| Chemical Compatibility | PBS, DI, 70% ethanol, dilute bleach |
Summit Instrument
Summit Instrument
EV isolation and concentration for high-capacity labs
Process up to 48 samples in parallel
Fully automated, including sample loading
Collect 1-to-3 fractions per sample
Plate-based ultrafiltration for concentration
Isolate 4 to 48 samples
Apex plates for the Summit
24-well SEC plates available in 4B and 6B
Equivalent performance to Apex columns, streamlining your isolation
Concentrate 4 to 48 samples
Altitude concentration plates for the Summit
Uses ultrafiltration membranes to concentrate EV fractions directly in a 24-well format
Designed to balance high recovery with reduction in liquid volume (down to 100 uL)
Direct collection of SEC fractions into Altitude UF plates during automated runs
Automate your workflow
Ascent vs. Summit: Choose Your Ideal Workflow
Compare features, throughput, and flexibility to find the best fit for your research needs.
 |
 |
|
Ascent |
Summit |
|
Best For |
Flexible sample prep | Large cohorts and standardized workflows |
Throughput Focus |
Moderate | High |
SEC Format |
Columns | Plates or Columns |
Sample Capacity |
1–8 samples | 4–48 samples |
Automation Level |
Manual sample loading required | Fully automated operation |
Fraction Collection |
1–12 fractions per sample | 1–3 fractions per sample |
Concentration Handling |
Performed externally | Integrated capability |
Footprint |
Compact | Larger, designed for scale |
Summit Instrument Specifications
| Units | Imperial | Metric |
| Dimensions (WxDxH) | 36” x 27” x 30” | 91 x 69 x 76 cm |
| Weight | 133 lbs. | 50 Kg |
| Bottle capacity | 1 U.S. gallons | 4 L |
| User Interface | 5.6” | 14.3mm |
| Environmental temperature | 50°F-75°F | 10°C -24°C |
| Relative humidity | 30−80% RH | |
| Input power | 100~240 VAC (50−60 Hz) | |
| Power supply unit | 24 VDC, 5 A, 120 W | |
| Connectivity | USB 3.1 | |
Atlas ELISA
Atlas ELISA Kits
Easy and reliable EV quantification assays
Run direct from biofluid or SEC fractions
Validated in plasma, serum, CSF, urine, cell culture media
Only 50 μL of sample – no need to purify or concentrate
Run 8 wells at a time or the full 96-well plate
1 incubation step
15-minutes hands on time
The Atlas EV ELISA is available in two formats:
◼︎ Absorbance ELISA: Simple and reliable EV yield and purity assessment using standard plate readers.
◼︎ Chemiluminescence (Lumi) ELISA: High sensitivity, wide dynamic range, and customizable capture (Homebrew options) for profiling disease-relevant markers.
| Atlas Human EV | |
| Dynamic range | 2 logs |
| Limit of detection | 1e8 |
| Reader compatibility | Absorbance |
| Custom targets |
Incubation
Add to the well plate
Dilute sample
Antibody mix
Wash Step
5X with Wash Buffer
Signal Generation
Add to the well-plate
100 uL TMB solution (5-10min)
100 uL Stop Solution
Signal Readout
Measure absorbance with plate reader
Human EV ELISA
Validated in :
Plasma
Serum
CSF
Urine
Cell culture media
Atlas Lumi ELISA
High-sensitivity, high-dynamic range ELISA for EV biomarker quantification
Technical information
The Atlas Human EV ELISA is an immunoassay to measure intact extracellular vesicles (EVs) in plasma, serum, urine, cell culture media, or cerebrospinal fluid (CSF).
This kit contains enough reagents for 96 wells.
| Absorbance | Chemiluminescence (Lumi) | |
| Reactive species | Human | Human |
| Well format | 8 x 12 strips | 8 x 12 strips |
| Dynamic range | 1e8 – 1e10 particles / mL | 1e7 - 1e11 particles / mL |
| Assay reproducibility (CV) | 5.5% | 5.75% |
| Limit of detection (LOD) | 1e8 particles / mL | 1e7 particles / mL |
| Lower limit of quantification (LLOQ) | 3.5e8 particles / mL | 1.4e7 particles / mL |
| Sample types | Plasma Serum Urine CSF Cell culture media |
Plasma Serum Urine CSF Cell culture media |
Atlas ELISA Reader
Compact, high-performance absorbance reader
Measure 96 wells at once
Get results in seconds
Space-saving format