C-NTA 나노입자추적분석기 ZetaViewⓇ
나노 입자가 브라운 운동이나 전기 영동에 의해 움직이는 궤적을 추적하여 Stokes-Einstein equation에 의해 입자의 크기(Size)를 분석하고,
mL당 입자의 개수(Concentration)와 제타 전위(Zeta Potential)를 분석하는 시스템 입니다.
제품 특징
1. 기기 한 대에 4개 레이저 동시 장착 가능 (QUATT)2. 1 Click Auto Focusing - 사용자간 편차 없음3. 여러 SOP를 레시피화하여 1 Click으로 Multi SOP 분석4. Sample Cell 전체 스캐닝 농도 분석 (220nl)5. 제타 전위(Zeta Potential) 분석6. Intensity 차이로 한 샘플 내 혼합 입자 크기 및 비율을 구분하여 분석7. Pump를 통한 Sample Cell 자동 세척8. Colocalization 이중 염색 입자 분석(추가 옵션)
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VIDEO
모델
MONO ZetaViewⓇ Evolution (PMX-140)
· Laser 1개 장착
· 자동 형광 필터
· 동일 샘플 내 1개의 형광 입자 분석 가능
TWIN ZetaViewⓇ Evolution (PMX-240)
· 한 기기에 2개의 Laser 장착
· 자동 형광 필터
· 동일 샘플 내 2개의 형광 입자 분석 가능
· Colocalization 옵션 추가 가능
QUATT ZetaViewⓇ Evolution (PMX-440)
· 한 장비에 Laser 4개 동시 장착
· 자동 형광 필터
· 동일 샘플 내 4개의 형광 입자 분석 가능
· Colocalization 옵션 추가 가능
MONO ZetaViewⓇ (PMX-130)
· Laser 1개 장착
· 자동 형광 필터
· 동일 샘플 내 1개의 형광 입자 분석 가능
TWIN ZetaViewⓇ (PMX-230)
· 한 기기에 2개의 Laser 장착
· 자동 형광 필터
· 동일 샘플 내 2개의 형광 입자 분석 가능
QUATT ZetaViewⓇ (PMX-430)
· 한 장비에 Laser 4개 동시 장착
· 자동 형광 필터
· 동일 샘플 내 4개의 형광 입자 분석 가능
레이저 파장별 형광 Dye
Laser
Dyes
Photostability
405 nm
Alexa® Fluor 405
+
Alexa® Fluor 430
++
Quantum dots
+++
Cell tracker violet
++
488 nm
Alexa® Fluor 488
+++
Atto 488
+++
PE, PE-Cy5
++
FITC
---
Rhodamine B
+
Calcein AM
+
CMG, PKH67, DIO
+++/++/++
GFP, eGFP
+/++
Laser
Dyes
Photostability
520 nm
Alexa® Fluor 546
+++
DyLightTM 550
+++
Alexa Fluor 555
+++
PE, PKH26 / PE-Cy5
+/++
Cy3
++
CMO
+++
RFP
+++
640 nm/
Alexa® Fluor 647
++
APC
++
Cy5
+++
CMDR, DIL
+++/++
F-NTA Kit for Zetaview ® Particle Metrix사의 F-NTA Kit는 CD9, CD63 및 CD81에 높은 결합 친화력을 가지고 있으며 NTA 응용 분아에서 EV 김출에 최적화되어 있습니다.
Easy EV Detecion with PAN EV staining
Customized for F-NTA
High binding affinity
Bright and stable fluorescence
3 Color conjugations
Matching controls
Matching exosome standards
x40 Zetaview Evolution 상세 사양
Measurement principle in scatter and fluorescence
Measurement principle in scatter and fluorescence
Size
Motorized scanning Nanoparticle Scanning Analysis (NTA) for measurement of up to 39 subvolumes per sample (129nl measurement
volume)
Concentration
Concentration Scanning Technology or NTA (220nl or 129nl measurement volume)
Zeta potential
Video based electrophoretic mobility tracking
Colocalization*
NTA based 2 channels overlay
Data management
Data management
Software
ZetaSphere control software featuring measurement of size, concentration, zeta potential and colocalization* in scatter and
fluorescence
Quality control
Integrated instrument performance check
Outlayer control by automatic Grubbs statistical analysis of measurement data
Database event logging for data integrity
Live monitoring of particle size and concentration, temperature, scattering intensity conductivity, particle drift and signal to noise ratio
Predefined measurement settings for several applications which are fully customizable
Data output
PDF multiparameter sample reporting
PNG
CSV
FCS
Physical characteristics
Physical characteristics
Dimensions
W x D x H : 20cm x 30cm x 25cm (excluding computer)
Electrical supply
90V - 240V; 47 - 63Hz; 50VA
Weight
13.5kg (excluding computer)
Power consumption
Hardware
Hardware
Instrument
90° laser scattering video microscope with x10 magnification for maximized sample volume and highest statistics
Four simultaneous aligned, software controlled lasers for use in scatter and fluorescence
Software controlled 12 positions fluorescence filter wheel for scatter and 11 fluorescence channels
Software controlled 2 pumps for liquid transport and sample dosing
Designed for automated sample loading
Automated alignment and focusing of laser and microscope
External temperature range : 5°C to 45°C
Sample temperature control via peltier element from RTP -5°C to 55°C with automated due point sensing
Camera
High sensitive CMOS camera with 1280x960 pixels
Variable frame rate from 2Hz to 60Hz for optimal resolution and fast acquisition
Lasers
Quatt lasers design with 405nm (130mW), 488nm (40mW), 520nm (80mW) and 640nm (130mW)
Pulse duration each laser : 0.1ms up to continuous
Filters
Software controlled automated 12 positions filter wheel equipped with 4 fluorescence emission long pass filters at 430nm, 500nm,
550nm and 660nm cut-off
customized emission filters available on request
Measurement cell
Quartz class cuvette for low protein binding
Tool free access for quick and simple cleaning process
Computer
i5 Asus® NUC Mini PC (i7 optional)
1TB SSD hard drive
Windows 11 Professional
Keyboard and mouse
Monitor
Measurement specifications
Measurement specifications
Size
10 ~ 1,000nm (dependent on sample and laser)
Accuracy : ±5% (for 100nm polystyrene latex)
Concentration
Concentration range : 105 ~ 109 particles/ml
Accuracy : ±5% (for 100nm polystyrene latex)
Fluorescence
Concentration range : 105 ~ 109 particles/ml
Sensitivity level : <20AF488 molecules
Zeta potential
Working range : -500mV ~ +500mV
Concentration range : 106 ~ 1010 particles/ml
Conductivity range : 3μs/cm ~ 15ms/cm
Reproducibility : ±2mV (Zeta potential standard)
General
Minimum sample quantity : 500μl of sample at 105 particles/ml
pH range : 1 ~ 13
Reference material
Nominal 100nm size and concentration reference suspension
Nominal 100nm or 200nm reference suspensions for fluorescence
x30 Zetaview 상세 사양
상세사양
Measurement principles
Micro-electrophoresis zeta potential and Brownian diffusion size, particle
concentration by video frame assessment
Optical layout
Laser scattering video microscope with individual particle tracking.
Auto-alignment and auto-focusing design
Measurement cell
and cassette
Fused silica channel, slide-in cassette fitting onto 2 fluidic ports for
rinsing and subvolume transport
Applied cell voltage
-24 V, + 24 V for zeta potential, 0 V for size
Optical System
Microscope objective x 10 and SCMOS camera, 640x480 px, 30 and 60 fps
Laser type depending on application
Zeta potential range
Range of detectable
particle size
10nm ~ 1,000nm for particle size determination
20nm ~ 5,000nm for zeta potential determination
Lower and upper limits dependant on sample and laser
pH-range
Temperature range
Temperature control
5 ~ 45°C outside temperature
RT -5°C, up to 45°C
Conductivity range
Internal control - outputs
Temperature, conductivity, electric field, drift
Trueness of measurement
± 4 mV in zeta potential; ± 5 nm for a 100 nm PS Latex
Reproducibility
± 2 mV in zeta potential; ± 2 nm for a 100 nm PS Latex
Sample
Aqueous and polar media based dispersions, minimum 500 μL
Sample concentration range
105 ~ 109 particles/mL for size measurements,
106 ~ 1010 particles/mL for zeta potential measurements
Test standards
Auto-alignment and daily check suspensions to dilute
Electrical supply
90 ~ 240 V, 47 ~ 63 Hz, 50 VA
Laser safety
Instrument protection to safety class I. Laser inside housing: safety class 3B,
switched off for access to the measurement cell.
External dimensions
20 (W) x 30 (D) x 25 (H) cm
Weight
Main unit 8.5 kg, PC extra
Measurement software
System control, auto-alignment. Tracking of individual particles.
Zeta potential distributions, size distributions, particle counting.
Profile and averaging functions. Export and report functions.
Theory
Conversion of measured electrophoretic mobility into zeta
potential according to Smoluchowski equation.
Size distribution following Stokes Einstein formula
Material parameters
Viscosity and dielectric constant, for water tabulated
Data management
Video files, txt files, pdf reporting, single and overlay output
