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Particle Metrix

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Particle Metrix

Particle Metrix
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C-NTA 나노입자추적분석기 ZetaViewⓇ

나노 입자가 브라운 운동이나 전기 영동에 의해 움직이는 궤적을 추적하여 Stokes-Einstein equation에 의해 입자의 크기(Size)를 분석하고, mL당 입자의 개수(Concentration)와 제타 전위(Zeta Potential)를 분석하는 시스템입니다.
형광 필터를 통해 Fluorescence Particles의 Size와 Concentration을 분석함으로써 전체 입자 중 형광 입자의 비율을 계산할 수 있습니다.

제품 특징

  • 1. 기기 한 대에 4개 레이저 동시 장착 가능 (QUATT)
  • 2. 1 Click Auto Focusing - 사용자간 편차 없음
  • 3. 여러 SOP를 레시피화하여 1 Click으로 Multi SOP 분석
  • 4. Sample Cell 전체 스캐닝 농도 분석 (220nl)
  • 5. 제타 전위(Zeta Potential) 분석
  • 6. Intensity 차이로 한 샘플 내 혼합 입자 크기 및 비율을 구분하여 분석
  • 7. Pump를 통한 Sample Cell 자동 세척
  • 8. Colocalization 이중 염색 입자 분석(추가 옵션)

모델 추천구성

MONO ZetaViewⓇ Evolution (PMX-140)

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  • · Laser 1개 장착
  • · 자동 형광 필터
  • · 동일 샘플 내 1개의 형광 입자 분석 가능

TWIN ZetaViewⓇ Evolution (PMX-240)

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  • · 한 기기에 2개의 Laser 장착
  • · 자동 형광 필터
  • · 동일 샘플 내 2개의 형광 입자 분석 가능
  • · Colocalization 옵션 추가 가능

QUATT ZetaViewⓇ Evolution (PMX-440)

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  • · 한 장비에 Laser 4개 동시 장착
  • · 자동 형광 필터
  • · 동일 샘플 내 4개의 형광 입자 분석 가능
  • · Colocalization 옵션 추가 가능
제품 상세 보기
제품 브로슈어 다운로드

레이저 파장별 형광 Dye

Laser Dyes Photostability
405 nm Alexa® Fluor 405 +
Alexa® Fluor 430 ++
Quantum dots +++
Cell tracker violet ++
488 nm Alexa® Fluor 488 +++
Atto 488 +++
PE, PE-Cy5 ++
FITC ---
Rhodamine B +
Calcein AM +
CMG, PKH67, DIO +++/++/++
GFP, eGFP +/++
Laser Dyes Photostability
520 nm Alexa® Fluor 546 +++
DyLightTM 550 +++
Alexa Fluor 555 +++
PE, PKH26 / PE-Cy5 +/++
Cy3 ++
CMO +++
RFP +++
640 nm/
660 nm		 Alexa® Fluor 647 ++
APC ++
Cy5 +++
CMDR, DIL +++/++

F-NTA Kit for ZetaView ®

Particle Metrix사의 F-NTA Kit는 CD9, CD63 및 CD81에 높은 결합 친화력을 가지고 있으며 NTA 응용 분야에서 EV 검출에 최적화되어 있습니다.
488nm, 520nm, 640nm 레이저에 최적화된 3가지 밝은 형광 조합으로 제공됩니다.
항체는 ZetaView®에 최적화된 염색 프로토콜 및 기기 설정과 함께 제공되므로 F-NTA 기반 CD9, CD63, CD81 검출이 용이합니다.

  • Easy EV Detecion with PAN EV staining
  • Customized for F-NTA
  • High binding affinity
  • Bright and stable fluorescence
  • 3 Color conjugations
  • Matching controls
  • Matching exosome standards
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제품 브로슈어 다운로드

x40 ZetaView Evolution 상세 사양

Measurement principle in scatter and fluorescence
Measurement principle in scatter and fluorescence
Size
  • Motorized scanning Nanoparticle Scanning Analysis (NTA) for measurement of up to 39 subvolumes per sample (129nl measurement volume)
Concentration
  • Concentration Scanning Technology or NTA (220nl or 129nl measurement volume)
Zeta potential
  • Video based electrophoretic mobility tracking
Colocalization*
  • NTA based 2 channels overlay
Data management
Data management
Software
  • ZetaSphere control software featuring measurement of size, concentration, zeta potential and colocalization* in scatter and fluorescence
Quality control
  • Integrated instrument performance check
  • Outlayer control by automatic Grubbs statistical analysis of measurement data
  • Database event logging for data integrity
  • Live monitoring of particle size and concentration, temperature, scattering intensity conductivity, particle drift and signal to noise ratio
  • Predefined measurement settings for several applications which are fully customizable
Data output
  • PDF multiparameter sample reporting
  • PNG
  • CSV
  • FCS
Physical characteristics
Physical characteristics
Dimensions
  • W x D x H : 20cm x 30cm x 25cm (excluding computer)
Electrical supply
  • 90V - 240V; 47 - 63Hz; 50VA
Weight
  • 13.5kg (excluding computer)
Power consumption
  • max. 30W
Hardware
Hardware
Instrument
  • 90° laser scattering video microscope with x10 magnification for maximized sample volume and highest statistics
  • Four simultaneous aligned, software controlled lasers for use in scatter and fluorescence
  • Software controlled 12 positions fluorescence filter wheel for scatter and 11 fluorescence channels
  • Software controlled 2 pumps for liquid transport and sample dosing
  • Designed for automated sample loading
  • Automated alignment and focusing of laser and microscope
  • External temperature range : 5°C to 45°C
  • Sample temperature control via peltier element from RTP -5°C to 55°C with automated due point sensing
Camera
  • High sensitive CMOS camera with 1280x960 pixels
  • Variable frame rate from 2Hz to 60Hz for optimal resolution and fast acquisition
Lasers
  • Quatt lasers design with 405nm (130mW), 488nm (40mW), 520nm (80mW) and 640nm (130mW)
  • Pulse duration each laser : 0.1ms up to continuous
Filters
  • Software controlled automated 12 positions filter wheel equipped with 4 fluorescence emission long pass filters at 430nm, 500nm, 550nm and 660nm cut-off
  • customized emission filters available on request
Measurement cell
  • Quartz class cuvette for low protein binding
  • Tool free access for quick and simple cleaning process
Computer
  • i5 Asus® NUC Mini PC (i7 optional)
  • 1TB SSD hard drive
  • Windows 11 Professional
  • Keyboard and mouse
Monitor
  • LED screen
Measurement specifications
Measurement specifications
Size
  • 10 ~ 1,000nm (dependent on sample and laser)
  • Accuracy : ±5% (for 100nm polystyrene latex)
Concentration
  • Concentration range : 105 ~ 109 particles/ml
  • Accuracy : ±5% (for 100nm polystyrene latex)
Fluorescence
  • Concentration range : 105 ~ 109particles/ml
  • Sensitivity level : <20AF488 molecules
Zeta potential
  • Working range : -500mV ~ +500mV
  • Concentration range : 106 ~ 1010 particles/ml
  • Conductivity range : 3μs/cm ~ 15ms/cm
  • Reproducibility : ±2mV (Zeta potential standard)
General
  • Minimum sample quantity : 500μl of sample at 105 particles/ml
  • pH range : 1 ~ 13
Reference material
  • Nominal 100nm size and concentration reference suspension
  • Nominal 100nm or 200nm reference suspensions for fluorescence
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